What are the disadvantages of stored isolates of marine bacteria?

1. What are the disadvantages of stored isolates of marine bacteria?2. Briefly describe redox potentials an environmental factor.3. What is the rationale behind the pyramid of numbers?4. Nutrient limited medium can be used for the isolation of which groups of bacteria?5. Which chemicals can be used for the estimation of microbial biomass of the following groups of microorganisms?a. Total microbial community.b. Marine bacteriac. Algae and cyanobacteriad. Fungi and prokaryotes6. a. which are the two major divisions of marine environment?7. The oceanic province is divided into how many regions and what are the characteristics of each region?8. What are the characteristics of deep sea sampling?9. What is the continental shelf and why is it importance?10. Describe microbial habitats in the marine environment.