Microbiology

Ceate a 2- to 3-page document in Microsoft Word for providing answers to questions in the following review sheets:

Support your responses with examples.

Cite any sources in APA format.

Week 1 – Review Sheet

Exercise1: Hanging-dropandwet-mountpreparations

1.   Howdoestruemotilitydifferfrom Brownianmovement?

2.   Whatmorphologicalstructureis responsibleforbacterialmotility?

3.   Whyisa wetpreparationdiscardedindisinfectantsolutionorbiohazardcontainer?

4.   Whatis thevalueofahanging-droppreparation?

5.   Whatis thevalueofa wet-mountpreparation?

   Exercise2: Simplestains

1.   Defineacidicandbasicdyes.Whatis thepurposeofeach?

2.   Whatis thepurposeoffixingaslidethatis tobe stained?

3.   Whyarethespecimenstobestainedsuspendedinsterilesalineordistilledwater?

4.   Howdoesastainedpreparationcomparewithahangingdropforstudyingthemorphologyand motilityofbacteria?

5.   Listat leastthreetypesofbacteriawhosenamesreflecttheirshapesandarrangements,and statethemeaningof eachname.

   Exercise3:Gramstain

1.   Whatis thefunctionoftheiodinesolutionintheGramstain?If itwereomitted,howwouldstaining resultsbeaffected?

2.   Whatis thepurposeofthealcoholsolutionintheGramstain?

3.   Whatcounterstainisused?Whyis itnecessary?Couldcolorsotherthanredbeused?

4.   Whatis theadvantageoftheGramstainoverasimplestainsuchasmethyleneblue?

5.   Inwhatkindofclinicalsituationwouldadirectsmearreportfrom thelaboratorybeof urgent importance?

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Microbiology

Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following review sheets:

Support your responses with examples.

Cite any sources in APA format.

Week 3 – Review Sheet

Exercise4: Somemetabolicactivitiesofbacteria

1.Whatis thecolorof phenolredatanacidpH?

2.Whatis thefunctionofaDurhamtube?

3.Whyisiodineusedtodetectstarchhydrolysis?

4.Howisindoleproducedin SIMmedium?Howis itdetected?

5.Howis hydrogensulfidedemonstratedinthismedium?

Exercise 5: Fungi, yeasts and molds

1.Whatis theadvantageof viewingmoldstructuresinatransparenttapepreparation?

2.Whatfunguscanbe identifiedreliablybyusingthegermtubetest?

3.Namethreestainsorreagentsthatmaybeusedtofacilitatethemicroscopicdetectionoffungiinclinical samples.

4.Whatis themainadvantageof usingtheslideculturetechniqueforidentifyingmolds?

5.Whatis anopportunisticpathogen?Namethreefungalspecimens.

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